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multisite gateway three-fragment vector construction kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multisite gateway three-fragment vector construction kit
    Multisite Gateway Three Fragment Vector Construction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multisite+gateway+three-fragment+vector+construction+kit/pm39160276-178-28-34
    Average 90 stars, based on 1 article reviews
    multisite gateway three-fragment vector construction kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Unveiling stem cell induction mechanisms from spatiotemporal cell-type-specific gene regulatory networks in postembryonic root organogensis
    Article Snippet: J0121 , J2661 , pWOX5::ER-GFP , pHB53:NLS-3xmCherry and pSKP2Bs::NLS-3x-mCherry lines were used in this study. dpGreen BarT pSCR:ER:3xmCherry construct was generated using MultiSite Gateway Three-Fragment Vector Construction Kit (Invitrogen) from previously generated pDONOR plasmids , and together with the dpGreen BarT pHB53::NLS-3x-mCherry contruct introgressed into the pWOX5::ER-GFP line using the floral dip method to generate the pHB53w::NLS-3x-mCherry pWOX5::ER-GFP and pSCR::NLS-3x-mCherry pWOX5::ER-GFP lines.

    Plasmid Preparation:

    Article Title: Analysis of Brain, Blood, and Testis Phenotypes Lacking the Vps13a Gene in C57BL/6N Mice
    Article Snippet: .. For targeting vector assembly, the three entry clones were recombined to a destination vector plasmid (pDEST-DT containing a cytomegalovirus enhancer/chicken beta-actin (CAG) promoter-driven diphtheria toxin gene) using the MultiSite Gateway Three-fragment Vector Construction Kit (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA). ..

    Article Title: Rufy3 is an adapter protein for small GTPases that activates a Rac guanine nucleotide exchange factor to control neuronal polarity
    Article Snippet: .. To target vector assembly, the three entry clones were recombined to a destination vector plasmid (pDEST-DT; containing a CAG promoter-driven diphtheria toxin gene) using a MultiSite Gateway Three-fragment Vector construction kit (Invitrogen). ..

    Article Title: PCPE-1, a brown adipose tissue-derived cytokine, promotes obesity-induced liver fibrosis.
    Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH, previously termed non-alcoholic steatohepatitis (NASH)), is a major complication of obesity that promotes fatty liver disease.. MASH is characterized by progressive tissue fibrosis and sterile liver inflammation that can lead to liver cirrhosis, cancer, and death.. The molecular mechanisms of fibrosis in MASH and its systemic control remain poorly understood.

    Clone Assay:

    Article Title: Analysis of Brain, Blood, and Testis Phenotypes Lacking the Vps13a Gene in C57BL/6N Mice
    Article Snippet: .. For targeting vector assembly, the three entry clones were recombined to a destination vector plasmid (pDEST-DT containing a cytomegalovirus enhancer/chicken beta-actin (CAG) promoter-driven diphtheria toxin gene) using the MultiSite Gateway Three-fragment Vector Construction Kit (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA). ..

    Article Title: Rufy3 is an adapter protein for small GTPases that activates a Rac guanine nucleotide exchange factor to control neuronal polarity
    Article Snippet: .. To target vector assembly, the three entry clones were recombined to a destination vector plasmid (pDEST-DT; containing a CAG promoter-driven diphtheria toxin gene) using a MultiSite Gateway Three-fragment Vector construction kit (Invitrogen). ..

    Article Title: PCPE-1, a brown adipose tissue-derived cytokine, promotes obesity-induced liver fibrosis.
    Article Snippet: Metabolic dysfunction-associated steatohepatitis (MASH, previously termed non-alcoholic steatohepatitis (NASH)), is a major complication of obesity that promotes fatty liver disease.. MASH is characterized by progressive tissue fibrosis and sterile liver inflammation that can lead to liver cirrhosis, cancer, and death.. The molecular mechanisms of fibrosis in MASH and its systemic control remain poorly understood.

    Construct:

    Article Title: The auxin signalling network translates dynamic input into robust patterning at the shoot apex
    Article Snippet: .. Gateway technology was then used to generate the different constructs following the multisite gateway three-fragment vector construction kit instructions (Invitrogen). ..

    Expressing:

    Article Title: C. elegans males optimize mate-choice decisions via sex-specific responses to multimodal sensory cues
    Article Snippet: Worm total RNA was purified from mixed-stage DR466 with a commercial kit (Qiagen catalog number 74134), followed by in vitro cDNA synthesis (Invitrogen catalog number 18080-051), as the template of bus-2 (isoform a) cDNA amplification. .. Other reagents used for the construction of expression plasmids include the MultiSite Gateway Three-fragment Vector Construction Kit (Invitrogen Catalog number 12537-023), DH5α competent cell (ThermoScientific catalog number 18265017). .. Plasmid sequences were confirmed by Sanger sequencing (Genewiz Inc, NJ, USA).



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